Insertion junction: LMJ.RY0402.163176_4

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.163176
Chromosome:chromosome 11
Location:2473116
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g475600 FAP362 Flagellar Associated Protein 362; (1 of 239) IPR016024 - Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGGTTCATACCGTACTCCGTTTGTAACCC
Internal bar code:GCATCTTCCTGGGGCGGGCAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:195
LEAP-Seq percent confirming:84.7645
LEAP-Seq n confirming:612
LEAP-Seq n nonconfirming:110
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:CGCCGCGAAAGATAAAGTAG
Suggested primer 2:CAAACTCTCATCGCCAAACA