| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.163182 |
| Chromosome: | chromosome 12 |
| Location: | 2999016 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g501000 | PPT1,TPT22,TPT20 | Putative UDP-GlcA transporter; (1 of 4) PTHR11132//PTHR11132:SF97 - SOLUTE CARRIER FAMILY 35 // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACACTAAGTCCAGCCGTGAACAAGGGC |
| Internal bar code: | AGGGCACTGGCCATGTGGAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 313 |
| LEAP-Seq percent confirming: | 99.7162 |
| LEAP-Seq n confirming: | 1054 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGTGAGAGGAGCGTGTC |
| Suggested primer 2: | CCACTCCTTCTTTTTCGCAG |