| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.163197 |
| Chromosome: | chromosome 10 |
| Location: | 2072374 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433100 | OBGE1,OBGE | Factor possibly involved in assembly of the ribosomal 50S assembly; (1 of 1) K03979 - GTP-binding protein (obg) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTATCACCGAGGTCGCCCTGGTCATGCTT |
| Internal bar code: | CACCAATAAATATGCCGCCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 357 |
| LEAP-Seq percent confirming: | 69.3061 |
| LEAP-Seq n confirming: | 779 |
| LEAP-Seq n nonconfirming: | 345 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGGTGAAGGGGTAGTTA |
| Suggested primer 2: | TGAAGAAATCTCGTGCGTTG |