Insertion junction: LMJ.RY0402.163208_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.163208
Chromosome:chromosome 11
Location:1722177
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g467784 (1 of 4) IPR006917//IPR011256 - SOUL haem-binding protein // Regulatory factor, effector binding domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGCAAGACCCCGCAAACCCCCACTCCTTG
Internal bar code:TTGGGGGTCATATTGGTCGCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:394
LEAP-Seq percent confirming:82.9158
LEAP-Seq n confirming:26213
LEAP-Seq n nonconfirming:5401
LEAP-Seq n unique pos:86

Suggested primers for confirmation by PCR

Suggested primer 1:TACAACGGTTACGCATCCAA
Suggested primer 2:GTACAGGGTCGCAAGGTGAT