Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.163210 |
Chromosome: | chromosome_16 |
Location: | 1629906 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g654300 | RSP23 | Radial spoke protein 23 | antisense | gene_edge/mRNA_edge/5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AATATGTGTAAGGTAACAGAGTGTGCAAGC |
Internal bar code: | TCTCGTTGCGCACCAGGAGCCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1030 |
LEAP-Seq percent confirming: | 94.5833 |
LEAP-Seq n confirming: | 681 |
LEAP-Seq n nonconfirming: | 39 |
LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCGTACCACTTAGGCTGCTC |
Suggested primer 2: | TAGCTTTCATGTGTCTGGCG |