Insertion junction: LMJ.RY0402.163237_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.163237
Chromosome:chromosome 7
Location:1732025
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g325715 (1 of 29) PF13637 - Ankyrin repeats (many copies) (Ank_4) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCCAGGCAGCTGGTGAGGGGGCACACAT
Internal bar code:TAACTGGAACGAACTCGGTTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:947
LEAP-Seq percent confirming:99.6482
LEAP-Seq n confirming:1133
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:ACTTCAAAACCGACTGGCAC
Suggested primer 2:AACAACACAGAGTCCAGGGC