| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.163245 |
| Chromosome: | chromosome 7 |
| Location: | 910666 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g318950 | (1 of 1) IPR000008//IPR000104//IPR003072 - C2 domain // Antifreeze protein, type I // Orphan nuclear receptor, NOR1 type | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGACCGACATTTAAACCTCTGCTGGCA |
| Internal bar code: | GCACTATCCGTCCGTACCCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 565 |
| LEAP-Seq percent confirming: | 99.5148 |
| LEAP-Seq n confirming: | 4102 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCTGTGTGTGTTGTGTCG |
| Suggested primer 2: | CTCACACAGCTGCTCACCTC |