| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.163285 |
| Chromosome: | chromosome 9 |
| Location: | 1833535 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395900 | CSV3 | Chlamydomonas-specific family protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCTTGCAGGTACCACTTCCCGCGGACGG |
| Internal bar code: | CACGGGTCGGTGCCGAGAGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 695 |
| LEAP-Seq percent confirming: | 98.0696 |
| LEAP-Seq n confirming: | 3099 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAATGACGCTTTAGCAGCC |
| Suggested primer 2: | TCCCTTTTTGTCCTCGTGTC |