| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.163304 |
| Chromosome: | chromosome 6 |
| Location: | 5234664 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g282200 | SNF7,VPS32 | Subunit of the ESCRT-III complex, SNF homolog | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGAGTGGTCATCCGCCACAGCTCCATCC |
| Internal bar code: | TTTCACTTGGCAAAAACACTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 473 |
| LEAP-Seq percent confirming: | 93.4239 |
| LEAP-Seq n confirming: | 1307 |
| LEAP-Seq n nonconfirming: | 92 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGCAGGAAGGAGCTAGT |
| Suggested primer 2: | TCAGTTTTGCAATCCAGCAG |