| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.163309 |
| Chromosome: | chromosome 3 |
| Location: | 1141717 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g149201 | (1 of 1) PTHR10641//PTHR10641:SF655 - MYB-LIKE DNA-BINDING PROTEIN MYB // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTAGCTGCAGCTGCGGACGGTGGCGAGG |
| Internal bar code: | CGTGTGATCGCGTACAACGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1285 |
| LEAP-Seq percent confirming: | 99.8462 |
| LEAP-Seq n confirming: | 649 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCACGCTGTCTATGTCGAG |
| Suggested primer 2: | ACACACACACACACACACCG |