Insertion junction: LMJ.RY0402.163311_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.163311
Chromosome:chromosome 4
Location:327952
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g217908 FAP79 Ankyrin Repeat Flagellar Associated Protein 79; (1 of 1) PTHR24133//PTHR24133:SF21//PTHR24198 - FAMILY NOT NAMED // ANKYRIN REPEAT-CONTAINING PROTEIN // ANKYRIN REPEAT AND PROTEIN KINASE DOMAIN-CONTAINING PROTEIN CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCCTGGAGCGCGACGTGGGCAGCCACGC
Internal bar code:CCGTAGGTGTCGCCGCGGCGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:253
LEAP-Seq percent confirming:99.2891
LEAP-Seq n confirming:419
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:AGCGCACATCCCTAGCTTTA
Suggested primer 2:TATCGAGGTTGTTGAGCGTG