Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.163321 |
Chromosome: | chromosome_16 |
Location: | 1627382 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g654300 | RSP23 | Radial spoke protein 23 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GGACAGACACGTATACAAGGCCTATGCTCC |
Internal bar code: | GGGTGATCGAGAGCAACGCGAA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 727 |
LEAP-Seq percent confirming: | 96.8203 |
LEAP-Seq n confirming: | 2710 |
LEAP-Seq n nonconfirming: | 89 |
LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCAACCTAGCAGGTCCTCAG |
Suggested primer 2: | TCTAGCAGCTTCCTACCGGA |