| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.163333 |
| Chromosome: | chromosome 1 |
| Location: | 3183731 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g019950 | DNJ7 | (1 of 1) PTHR24078:SF294 - DNAJ PROTEIN HOMOLOG 1; DnaJ-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTGTACCGTACGCATGGTCCCCTACTTA |
| Internal bar code: | AGTATAGTTGGTGCCTCGGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 45 |
| LEAP-Seq percent confirming: | 99.7445 |
| LEAP-Seq n confirming: | 2342 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTCACTTCCTCCTGCAC |
| Suggested primer 2: | ACGCACCCATTTAACTTTGC |