| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.163358 |
| Chromosome: | chromosome 12 |
| Location: | 1742446 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512600 | RPL18 | Ribosomal protein L18, component of cytosolic 80S ribosome and 6 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTTGGGAACATGGGCCATCCGGCTGTCG |
| Internal bar code: | GCGCCAGGATTATTGCTGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 704 |
| LEAP-Seq percent confirming: | 97.7876 |
| LEAP-Seq n confirming: | 884 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTCTGGCGATGGTGTAAC |
| Suggested primer 2: | AAGCTGACATTGATTTGGGG |