Insertion junction: LMJ.RY0402.163361_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.163361
Chromosome:chromosome 7
Location:1809339
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g325726 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTTGCTCGCCCAGACCATCGCGGTCCTGC
Internal bar code:CAGAGGGAGTCAAGAGACGATG

Confirmation - LEAP-Seq

LEAP-Seq distance:683
LEAP-Seq percent confirming:99.7625
LEAP-Seq n confirming:840
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:AACGCAACAAACCATGTCAA
Suggested primer 2:TGTACTTGGCTGCCTCTCCT