Insertion junction: LMJ.RY0402.163391_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.163391
Chromosome:chromosome 10
Location:473893
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g421000 (1 of 10) IPR000104//IPR001810 - Antifreeze protein, type I // F-box domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTTCACTTGTCTTGGTACGGCACTCACCG
Internal bar code:TGGTGCATCGGGAGATGTCAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:190
LEAP-Seq percent confirming:29.4645
LEAP-Seq n confirming:1381
LEAP-Seq n nonconfirming:3306
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CGGATGATCTGCAGCTAACA
Suggested primer 2:CGTTTCAATGCGGTAAAGGT