Insertion junction: LMJ.RY0402.163395_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.163395
Chromosome:chromosome 8
Location:902672
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre08.g361450 (1 of 1) IPR008867//IPR009836 - Thiazole biosynthesis // Protein of unknown function DUF1399 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCCACGCTCCACACTCGCCCACGATCCCG
Internal bar code:TAAATTACGGATGCCGATTGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:110
LEAP-Seq percent confirming:99.6812
LEAP-Seq n confirming:3127
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTCTCACATGAACGGCTA
Suggested primer 2:CAAAACCTTCAACAGCAGCA