Insertion junction: LMJ.RY0402.163403_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.163403
Chromosome:chromosome 9
Location:1107644
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre09.g400312 (1 of 5) IPR001680//IPR011047//IPR017986 - WD40 repeat // Quinonprotein alcohol dehydrogenase-like superfamily // WD40-repeat-containing domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTGCCCTCCCCGTGTGCCTTGACCCCCT
Internal bar code:AACGTCCTGCGCGGGGAGAAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:269
LEAP-Seq percent confirming:99.9244
LEAP-Seq n confirming:1322
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCACATCGTCTCTCTGCT
Suggested primer 2:TACGAGAACGCTGTGAATGG