| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.163434 |
| Chromosome: | chromosome 9 |
| Location: | 7304314 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g412803 | CGL88 | predicted protein; (1 of 1) PTHR10383//PTHR10383:SF24 - SERINE INCORPORATOR // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGCCTGATAACTCATACGTCAATACAAC |
| Internal bar code: | CATACCTAGGCCCTACCGTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 683 |
| LEAP-Seq percent confirming: | 99.8099 |
| LEAP-Seq n confirming: | 2625 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAGAGGGGAAAGAGGAGG |
| Suggested primer 2: | CGGTGTGTGTGTGTGTGTGT |