Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.163449 |
Chromosome: | chromosome_6 |
Location: | 3935301 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g278187 | MOT4 | Predicted protein | antisense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTCGTGCTGGCGCCAGTCAACAGCGTCAGA |
Internal bar code: | GTAGACCATGCCGGGAAGCCAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 473 |
LEAP-Seq percent confirming: | 96.9585 |
LEAP-Seq n confirming: | 10265 |
LEAP-Seq n nonconfirming: | 322 |
LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GACTGAGGTTCTCCGAGTGC |
Suggested primer 2: | GTGTACGAGGACGAGTTCCC |