| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.163465 |
| Chromosome: | chromosome 12 |
| Location: | 3667436 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g514350 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGGGCCGGCTTTCCGGTCAAGCTCGCCT |
| Internal bar code: | ACGATAAGGGGGATAGGGAACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 241 |
| LEAP-Seq percent confirming: | 44.7033 |
| LEAP-Seq n confirming: | 1439 |
| LEAP-Seq n nonconfirming: | 1780 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCCAATACCGACTACGCC |
| Suggested primer 2: | GTGTGCGTTGAGTGGAGTGT |