| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.163468 |
| Chromosome: | chromosome 6 |
| Location: | 2887705 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g272150 | (1 of 1) 1.8.4.2 - Protein-disulfide reductase (glutathione) / Insulin reductase | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCATGCCAGTCAGCGCGCTTGGTCAGTG |
| Internal bar code: | CGCGCGTCCGTTAGCGCAGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 989 |
| LEAP-Seq percent confirming: | 99.8347 |
| LEAP-Seq n confirming: | 2416 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGATACCGTACCGCCACTG |
| Suggested primer 2: | TATACCAGGTTGCAGCTCCC |