| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.163482 |
| Chromosome: | chromosome 10 |
| Location: | 3257500 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443100 | (1 of 1) IPR001214//IPR023220 - SET domain // Type IV secretion system, VirB5-domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGTTGCCAGGAACGGGCTGCGGTTGGCG |
| Internal bar code: | AATGTGGCGCTTCAATAATTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1060 |
| LEAP-Seq percent confirming: | 94.8475 |
| LEAP-Seq n confirming: | 1399 |
| LEAP-Seq n nonconfirming: | 76 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCTGGCAGCAGGTGATTT |
| Suggested primer 2: | GGAATGTTGTCGCGTACCTT |