| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.163528 |
| Chromosome: | chromosome 2 |
| Location: | 5219131 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105900 | (1 of 1) 3.1.1.7 - Acetylcholinesterase / True cholinesterase | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTTTAGTTTAGGAAGATGAATGCGCAC |
| Internal bar code: | GTTCTGCCTTGGGTGCGAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 553 |
| LEAP-Seq percent confirming: | 97.0103 |
| LEAP-Seq n confirming: | 80017 |
| LEAP-Seq n nonconfirming: | 2466 |
| LEAP-Seq n unique pos: | 200 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACCGTAATGCAGGTGGCT |
| Suggested primer 2: | TTTATCCCGACCGTAACGAG |