| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.163539 |
| Chromosome: | chromosome 13 |
| Location: | 4407101 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g603100 | GT90-48,GT90F48 | GT90 family protein 48; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCCCCAGAAAGGGCCCCATGGTCTGAC |
| Internal bar code: | GCATCGACGGCATGTATGACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 976 |
| LEAP-Seq percent confirming: | 98.3232 |
| LEAP-Seq n confirming: | 11317 |
| LEAP-Seq n nonconfirming: | 193 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACTCACGGGCATTAGGT |
| Suggested primer 2: | GGTTACGGACCAGTCTGCAT |