| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.163561 |
| Chromosome: | chromosome 2 |
| Location: | 1226680 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g082350 | CUTA1,CUT1 | (1 of 1) K03926 - periplasmic divalent cation tolerance protein (cutA); Copper-binding protein CutA | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAACAATGGCAGCGCGCGGGCGTTCCGGC |
| Internal bar code: | TCCGGAAAGGAGTTACAAGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 267 |
| LEAP-Seq percent confirming: | 99.7248 |
| LEAP-Seq n confirming: | 3986 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCATGCTTGGGTTCGATT |
| Suggested primer 2: | GGAGGCGCGTTAAACAGTAG |