| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.163562 |
| Chromosome: | chromosome 1 |
| Location: | 4415333 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g029900 | (1 of 3) 3.6.3.17 - Monosaccharide-transporting ATPase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCAATTTATGTTCAAGCCGTTGCACCA |
| Internal bar code: | CAACTGGAATCGAGGCCGAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 832 |
| LEAP-Seq percent confirming: | 99.8204 |
| LEAP-Seq n confirming: | 5001 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAGCTTCATGCTGTCGTC |
| Suggested primer 2: | AATAGACCAGGTGCACCGAC |