Insertion junction: LMJ.RY0402.163590_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.163590
Chromosome:chromosome 2
Location:3879962
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g096000 (1 of 14) 3.6.3.25 - Sulfate-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGTATCAGCACCTGTATGTCAAGAGGAAC
Internal bar code:GCCATCAAGCTTGACCACACAG

Confirmation - LEAP-Seq

LEAP-Seq distance:969
LEAP-Seq percent confirming:97.1798
LEAP-Seq n confirming:2481
LEAP-Seq n nonconfirming:72
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:AACATCGCAGTCTTCTGGCT
Suggested primer 2:CACACGGTCACTCAACAACC