| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.163598 |
| Chromosome: | chromosome 16 |
| Location: | 5729786 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g678850 | (1 of 1) 2.3.3.10 - Hydroxymethylglutaryl-CoA synthase / Hydroxymethylglutaryl coenzyme alpha-condensing enzyme | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACTAACGTACTAGCATCTTTGCTATGATA |
| Internal bar code: | AGTTAGTCACTCTACCAATGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 768 |
| LEAP-Seq percent confirming: | 99.3384 |
| LEAP-Seq n confirming: | 6156 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGGCACTACCTGGAATGG |
| Suggested primer 2: | GGCTTGGCTAGGACTCACTG |