| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.163646 |
| Chromosome: | chromosome 16 |
| Location: | 6074448 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676050 | ARP3 | (1 of 1) K18584 - actin-related protein 3 (ACTR3, ARP3); Actin-related protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGAAAGGCCGCGGATGCTGTAACCTGCC |
| Internal bar code: | GAGCAGTCCGGGATAGGGCTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1018 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 78 |
| LEAP-Seq n nonconfirming: | 78 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATCGGCAACTTAAAGAGC |
| Suggested primer 2: | ACCCCTTCAAAATATTGGGC |