Insertion junction: LMJ.RY0402.163648_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.163648
Chromosome:chromosome 3
Location:4328719
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g174950 TGL7 (1 of 3) PTHR21493//PTHR21493:SF87 - CGI-141-RELATED/LIPASE CONTAINING PROTEIN // SN1-SPECIFIC DIACYLGLYCEROL LIPASE BETA; Putative triacylglycerol lipase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCACCACGATTACGCCACCACACCCATTT
Internal bar code:CAAGTGTTGGGGCCTCGGCCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:595
LEAP-Seq percent confirming:97.2621
LEAP-Seq n confirming:1421
LEAP-Seq n nonconfirming:40
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGGTGGTGATGAGGAGTT
Suggested primer 2:GAACACCATTCATCCATCCA