Insertion junction: LMJ.RY0402.163655_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.163655
Chromosome:chromosome 8
Location:635227
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g359950 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTAATGCAACGGCTGCACGCACACAGGG
Internal bar code:TTGAAACGGGGGGGGTACCTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:979
LEAP-Seq percent confirming:99.1916
LEAP-Seq n confirming:2822
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGTCAAGGTGGGTTCATC
Suggested primer 2:GTCTTGGCTGCTAGGTTTGC