Insertion junction: LMJ.RY0402.163700_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.163700
Chromosome:chromosome 1
Location:2032506
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g010880 (1 of 2) K18598 - echinoderm microtubule-associated protein-like 6 (EML6) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACACACACGCACGCCCACAGGCTAGTG
Internal bar code:GCCTCCGAAAGGAGAGCAGTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:190
LEAP-Seq percent confirming:77.492
LEAP-Seq n confirming:241
LEAP-Seq n nonconfirming:70
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTGACTCTTGGCACCTTC
Suggested primer 2:GAGGTGTCGTGCAAGCAGTA