| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.163703 |
| Chromosome: | chromosome 10 |
| Location: | 1835338 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g431200 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGGAATGAGATACGCGTTCTGTAGGCGC |
| Internal bar code: | CAGGAAAATCATATTCTCGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 708 |
| LEAP-Seq percent confirming: | 99.5947 |
| LEAP-Seq n confirming: | 2703 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGGTGAAACGGGAAACTA |
| Suggested primer 2: | ACCTGAATCGTTCAAGGCAA |