| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.163714 |
| Chromosome: | chromosome_13 |
| Location: | 3734350 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g589350 | FBB6 | Flagellar/basal body protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGGGGGCCCACTGCGACTGGGCTCACTCCG |
| Internal bar code: | CGGGTAGAGCTAGAACTATAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 592 |
| LEAP-Seq percent confirming: | 98.2601 |
| LEAP-Seq n confirming: | 1186 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTGGCGCTACAGTGACAA |
| Suggested primer 2: | GACTGGTGGACGAGGGACTA |