Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.163714 |
Chromosome: | chromosome_13 |
Location: | 3734370 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g589350 | FBB6 | Flagellar/basal body protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AAGCCGTGGGTTTCATCGTCTCCGTATTAC |
Internal bar code: | GTACGCCTAGTTGCAGCGAAGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 675 |
LEAP-Seq percent confirming: | 94.5822 |
LEAP-Seq n confirming: | 2060 |
LEAP-Seq n nonconfirming: | 118 |
LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GACTGGTGGACGAGGGACTA |
Suggested primer 2: | CAGTGAGAGGTCCTTGGAGG |