| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.163755 |
| Chromosome: | chromosome_12 |
| Location: | 3384303 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g497000 | FAP14 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCTCTATGCCCGCCACAGCCAGAGCCTTGC |
| Internal bar code: | CCCGATTGTGCATCTGATCCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 390 |
| LEAP-Seq percent confirming: | 99.83 |
| LEAP-Seq n confirming: | 5285 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTTCGCTGTCCAAGCAG |
| Suggested primer 2: | GCCCATCCTGGACTTGAGTA |