Insertion junction: LMJ.RY0402.163761_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.163761
Chromosome:chromosome_6
Location:7983188
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre06.g303800 sense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):AGAGTCTGGCGCTCCCGCCATCCGTCCATT
Internal bar code:TCCGTCGGCCTCGCTACCTGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:990
LEAP-Seq percent confirming:82.9675
LEAP-Seq n confirming:6464
LEAP-Seq n nonconfirming:1327
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:CACAGCTTTCGGTTCCTTTC
Suggested primer 2:GACTGTTTCCTGTCGGCATT