Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.163761 |
Chromosome: | chromosome_6 |
Location: | 7983188 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g303800 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AGAGTCTGGCGCTCCCGCCATCCGTCCATT |
Internal bar code: | TCCGTCGGCCTCGCTACCTGGT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 990 |
LEAP-Seq percent confirming: | 82.9675 |
LEAP-Seq n confirming: | 6464 |
LEAP-Seq n nonconfirming: | 1327 |
LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CACAGCTTTCGGTTCCTTTC |
Suggested primer 2: | GACTGTTTCCTGTCGGCATT |