| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.163773 |
| Chromosome: | chromosome 10 |
| Location: | 2371837 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g435200 | (1 of 4) IPR000533 - Tropomyosin | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAAGCTTTGCGGTTTCAGTGAGAAATAC |
| Internal bar code: | TTTTTGGTGGGGTACCACTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 131 |
| LEAP-Seq percent confirming: | 99.4764 |
| LEAP-Seq n confirming: | 570 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | GTTAGAGCTCGGCAAAAACG |