| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.163813 |
| Chromosome: | chromosome 10 |
| Location: | 3776822 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g446800 | (1 of 3) IPR016087 - Chalcone isomerase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGGTTTGAGGCGTATGTAAAACAGACTG |
| Internal bar code: | ACTAACATAACCTTAGAGGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 406 |
| LEAP-Seq percent confirming: | 99.6299 |
| LEAP-Seq n confirming: | 6191 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCACAGGAGCCAAAGAGC |
| Suggested primer 2: | TTAGGGAAGTCACCCTGTCG |