Insertion junction: LMJ.RY0402.163814_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.163814
Chromosome:chromosome 2
Location:1687971
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g085850 ARS6 Arylsulfatase; (1 of 19) 3.1.6.1 - Arylsulfatase / Sulfatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAGACCAGCAGCGACACATTCTACAGGGC
Internal bar code:ACTTTCTCAAGGGTTGCTACTT

Confirmation - LEAP-Seq

LEAP-Seq distance:878
LEAP-Seq percent confirming:99.6664
LEAP-Seq n confirming:9559
LEAP-Seq n nonconfirming:32
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:AAACCCGCGATATGTAGACG
Suggested primer 2:CGTAAGGCTGGAAGTGGAAG