| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.163828 |
| Chromosome: | chromosome_10 |
| Location: | 4559544 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g452400 | CSF1 | Predicted protein of CSF family | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGCACACCTCTCAGAAACCTCCCTGAGACT |
| Internal bar code: | TCCTGCAACCGCATGTGCAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 667 |
| LEAP-Seq percent confirming: | 99.6055 |
| LEAP-Seq n confirming: | 505 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCAGACCTCAGCCCTTCAC |
| Suggested primer 2: | ACAATCTGCTGGCACAACTG |