| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.163828 |
| Chromosome: | chromosome_11 |
| Location: | 2082850 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g469000 | FAP107 | Flagellar Associated Protein | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCCCCGCAATGCAAATATGCAATGTTGCAC |
| Internal bar code: | GACGGCCGTGTGCCCACTCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 992 |
| LEAP-Seq percent confirming: | 99.7238 |
| LEAP-Seq n confirming: | 722 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAATAACGTAGCCGATGC |
| Suggested primer 2: | TTTGTTGTGTCCATCTGGGA |