| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.163848 |
| Chromosome: | chromosome 2 |
| Location: | 8668060 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g142126 | FAP115 | Flagellar Associated Protein 115 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGATTGGGTAGTGTCAGACCCTGAGGT |
| Internal bar code: | TAACCTTTAAGTGTCCAGAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 950 |
| LEAP-Seq percent confirming: | 99.4355 |
| LEAP-Seq n confirming: | 2466 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGCTAGTAAACAGCGCCA |
| Suggested primer 2: | CGGAAGAGACTGCAGGTAGG |