Insertion junction: LMJ.RY0402.163848_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.163848
Chromosome:chromosome_2
Location:8668060
Confidence (%):73
Locus systematic id Locus common name Defline Orientation Feature
Cre02.g142126 FAP115 Flagellar Associated Protein sense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):GCTGGATTGGGTAGTGTCAGACCCTGAGGT
Internal bar code:TAACCTTTAAGTGTCCAGAAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:950
LEAP-Seq percent confirming:99.4355
LEAP-Seq n confirming:2466
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGCTAGTAAACAGCGCCA
Suggested primer 2:CGGAAGAGACTGCAGGTAGG