Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.163848 |
Chromosome: | chromosome_12 |
Location: | 4318599 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g519950 | FAP42 | Flagellar Associated Protein, adenylate/guanylate kinase-like | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ATGGCGAATCAACTAACGACAGCTCGTCAC |
Internal bar code: | CCCAGACAGGCCCATAATTATG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 316 |
LEAP-Seq percent confirming: | 99.8682 |
LEAP-Seq n confirming: | 1516 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGCGCTCAATAACCTCCAGA |
Suggested primer 2: | GGCCAACATGCTTGAAAAAT |