| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.163866 |
| Chromosome: | chromosome 14 |
| Location: | 2596677 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g625850 | MMP32 | Matrix metalloproteinase; (1 of 1) IPR003980//IPR008752 - Histamine H3 receptor // Peptidase M11, gametolysin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCCCGCTCCCCTGATCCCATTCCCATAC |
| Internal bar code: | CTCTGTTGGCTCTGTTTGGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 228 |
| LEAP-Seq percent confirming: | 99.7438 |
| LEAP-Seq n confirming: | 1168 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTACGCGTCCACTTCTTTC |
| Suggested primer 2: | AGAGACGCAAGTAAGGGCAA |