| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.163870 |
| Chromosome: | chromosome 13 |
| Location: | 2525511 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g580500 | (1 of 2) PTHR12847:SF3 - NECAP-LIKE PROTEIN CG9132 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAAGAACCGTGCGACAGGTTGGGCAGTC |
| Internal bar code: | TGTGGGACTCTCGGAGTGATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 704 |
| LEAP-Seq percent confirming: | 96.3686 |
| LEAP-Seq n confirming: | 6767 |
| LEAP-Seq n nonconfirming: | 255 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACACTACTGCCAAGACGC |
| Suggested primer 2: | GCAGCTGTGAAACCATCTCA |