Insertion junction: LMJ.RY0402.163882_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.163882
Chromosome:chromosome 4
Location:2620748
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g222800 LCID,LCID1 Low-CO2 inducible protein D CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGTCAGGTTGGCCTCCAGCGCCGAAGCA
Internal bar code:TCCGCTAGGGGCACAGATGACC

Confirmation - LEAP-Seq

LEAP-Seq distance:1112
LEAP-Seq percent confirming:99.858
LEAP-Seq n confirming:2812
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GAATACGTAGCGCTCCTTGC
Suggested primer 2:GCATGATTGCTCACCATGAC