Insertion junction: LMJ.RY0402.163887_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.163887
Chromosome:chromosome 3
Location:112031
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g143987 (1 of 1) IPR011016//IPR014048 - Zinc finger, RING-CH-type // Methylated-DNA-[protein]-cysteine S-methyltransferase, DNA binding 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAACGCCGGCAACGCGACTACTGGCGTGCA
Internal bar code:TGAAAATCCTGAGTAACTCGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0869565
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:2298
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCAACCATGTTTCACACAGC
Suggested primer 2:CGCCTAAACGTATCGTGGAT