| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.163897 |
| Chromosome: | chromosome_3 |
| Location: | 5077625 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g181150 | DLL1,ROC106,FLA14,RSP22,LC8 | Dynein arm light chain 8, LC8 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGTCAGTTTTCTATCAGTCCACCAAGGAGG |
| Internal bar code: | CGTAAGGGCAGGTGCTCGTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 798 |
| LEAP-Seq percent confirming: | 99.8273 |
| LEAP-Seq n confirming: | 578 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGATGGACGTTGTTGCGT |
| Suggested primer 2: | GGTTCAGCTAGGATGCGAAG |